EMDB EMD-3449

Single particle reconstruction
4.6Å resolution

RNA Polymerase I elongation complex with A49 tandem winged helix domain

Map released:
Last modified:
Overview of EMD-3449
Source organism: Baker's yeast [4932]
Fitted atomic model: 5m64
Related EM entries by publication: EMD-3446, EMD-3447, EMD-3448
Primary publication:
Molecular Structures of Transcribing RNA Polymerase I.
Tafur L, Sadian Y, Hoffmann NA, Jakobi AJ, Wetzel R, Hagen WJ, Sachse C, Muller CW
Mol. Cell 64 1135-1143 (2016)
PMID: 27867008

Function and Biology Details

Sample name: RNA Polymerase I elongation complex with A49 tandem winged helix domain
Ligand: ZINC ION
Nucleic acids: Non-template DNA, Template DNA, RNA
Proteins: RNA Polymerase I elongation complex with A49 tandem winged helix domain, Transcription scaffold, DNA-directed RNA polymerase I subunit RPA190, DNA-directed RNA polymerase I subunit RPA135, DNA-directed RNA polymerases I and III subunit RPAC1, DNA-directed RNA polymerase I subunit RPA14, DNA-directed RNA polymerases I, II, and III subunit RPABC1, DNA-directed RNA polymerases I, II, and III subunit RPABC2, DNA-directed RNA polymerase I subunit RPA43, DNA-directed RNA polymerases I, II, and III subunit RPABC3, DNA-directed RNA polymerase I subunit RPA12, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerases I and III subunit RPAC2, DNA-directed RNA polymerases I, II, and III subunit RPABC4, DNA-directed RNA polymerase I subunit RPA49, DNA-directed RNA polymerase I subunit RPA34

Experimental Information Details

Resolution: 4.6Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)