EMDB EMD-3385

Single particle reconstruction
3.9Å resolution

Cryo-EM structure of the human APC/C-Cdc20-Hsl1 complex

Map released:
Last modified:
Overview of EMD-3385
Source organisms:
Fitted atomic model: 5g04
Related EM entries by publication: EMD-3386, EMD-3387, EMD-3388, EMD-3389, EMD-3390
Primary publication:
Molecular mechanism of APC/C activation by mitotic phosphorylation
Zhang S, Chang L, Alfieri C, Zhang Z, Yang J, Maslen S, Skehel M, Barford D
NATURE 533 260-264 (2016)
PMID: 27120157

Function and Biology Details

Sample name: Recombinant human APC/C-Cdc20-Hsl1 complex
Proteins: Anaphase-promoting complex subunit 1, Anaphase-promoting complex subunit 2, Anaphase-promoting complex subunit 3, Anaphase-promoting complex subunit 4, Anaphase-promoting complex subunit 5, Anaphase-promoting complex subunit 6, Anaphase-promoting complex subunit 7, Anaphase-promoting complex subunit 8, Anaphase-promoting complex subunit 10, Anaphase-promoting complex subunit 11, Anaphase-promoting complex subunit 12, Anaphase-promoting complex subunit 13, Anaphase-promoting complex subunit 15, Anaphase-promoting complex subunit 16, Cell division cycle protein 20 homolog, Hsl1

Experimental Information Details

Resolution: 3.9Å
Resolution method: FSC 0.143, gold-standard
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI POLARA 300
Detector: FEI FALCON II (4k x 4k)