Single particle reconstruction
3.3Å resolution

Cryo-EM structure of human p97 bound to ATPgS (Conformation III)

Map released:
Last modified:
Overview of EMD-3299
Source organisms:
Fitted atomic model: 5ftn
Related EM entries by publication: EMD-3295, EMD-3296, EMD-3297, EMD-3298
Primary publication:
2.3 A resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition
Banerjee S, Bartesaghi A, Merk A, Rao P, Bulfer SL, Yan Y, Green N, Mroczkowski B, Neitz RJ, Wipf P, Falconieri V, Deshaies RJ, Milne JLS, Huryn D, Arkin M, Subramaniam S
SCIENCE 351 871-875 (2016)
PMID: 26822609

Function and Biology Details

Sample name: Full-length human p97 bound to ATPgS
Ligand: Adenosine 5 gamma-thio triphosphate
Protein: p97/VCP Transitional endoplasmic reticulum ATPase

Experimental Information Details

Resolution: 3.3Å
Resolution method: FSC 0.143, semi-independent
Applied symmetry: C6
Reconstruction software: FREALIGN
Detector: GATAN K2 SUMMIT (4k x 4k)