EMDB EMD-3220

Single particle reconstruction
3.7Å resolution

Structure of transcribing mammalian RNA polymerase II (EC3)

Map released:
Last modified:
Overview of EMD-3220
Source organisms:
Related EM entry by publication: EMD-3218, EMD-3219
Primary publication:
Structure of transcribing mammalian RNA polymerase II.
Bernecky C, Herzog F, Baumeister W, Plitzko JM, Cramer P
NATURE 529 551-554 (2016)
PMID: 26789250

Function and Biology Details

Sample name: bovine Pol II elongation complex
Nucleic acid: DNA-RNA synthetic construct
Proteins: DNA-directed RNA polymerase II, DNA-directed RNA polymerase II subunit GRINL1A

Experimental Information Details

Resolution: 3.7Å
Resolution method: FSC 0.143, gold-standard
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)