EMDB EMD-3146

Single particle reconstruction
4.1Å resolution

Mechanism of eIF6 release from the nascent 60S ribosomal subunit

Map released:
Last modified:
Overview of EMD-3146
Source organisms:
Fitted atomic model: 5anb
Related EM entry by publication: EMD-3145, EMD-3147
Primary publication:
Mechanism of eIF6 release from the nascent 60S ribosomal subunit
Weis F, Giudice E, Churcher M, Jin L, Hilcenko C, Wong CC, Traynor D, Kay RR, Warren AJ
NAT.STRUCT.MOL.BIOL. 22 914-919 (2015)
PMID: 26479198

Function and Biology Details

Sample name: Dictyostelium 60S carrying endogenous eIF6 with recombinant human SBDS and human EFL1
Proteins: Eukaryotic translation initiation factor 6, Shwachman-Bodian-Diamond syndrome protein, Elongation factor-like 1
Ribosome: 60S ribosomal subunit

Experimental Information Details

Resolution: 4.1Å
Resolution method: FSC 0.143, gold-standard
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)