EMDB EMD-30608

Single particle reconstruction
3.3Å resolution

Cryo-EM Structure of the Prostaglandin E Receptor EP4 Coupled to G Protein

Map released:
Last modified:
Overview of EMD-30608
Source organisms:
Fitted atomic model: 7d7m
To be published

Function and Biology Details

Sample name: PGE2-EP4-Gs-Gbeta1-Ggamma2-Nb35 complex
Ligand: (Z)-7-[(1R,2R,3R)-3-hydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl]-5-oxo-cyclopentyl]hept-5-enoic acid
Proteins: PGE2-EP4-Gs-Gbeta1-Ggamma2-Nb35 complex, Prostaglandin E2 receptor EP4 subtype,Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1,Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, Guanine nucleotide-binding protein G(s) subunit alpha isoforms short, nanobody Nb35, Prostaglandin E2 receptor EP4 subtype,Prostaglandin E2 receptor EP4 subtype, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short, nanobody Nb35

Experimental Information Details

Resolution: 3.3Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: OTHER