EMDB EMD-3047

Single particle reconstruction
3.46Å resolution

40S-eIF1A-eIF1 complex

Map released:
Last modified:
Overview of EMD-3047
Source organisms:
Fitted atomic model: 3jam
Related EM entries by publication: EMD-0057, EMD-0058, EMD-3048, EMD-3049, EMD-3050
Primary publication:
Conformational Differences between Open and Closed States of the Eukaryotic Translation Initiation Complex.
Llacer JL, Hussain T, Marler L, Aitken CE, Thakur A, Lorsch JR, Hinnebusch AG, Ramakrishnan V
MOL.CELL 59 399-412 (2015)
PMID: 26212456

Function and Biology Details

Sample name: 40S-eIF1-eIF1A preinitiation complex
Proteins: Eukaryotic initiation factor 1, Eukaryotic initiation factor 1A
Ribosome: Ribosome small subunit

Experimental Information Details

Resolution: 3.46Å
Resolution method: FSC 0.143, gold-standard
Applied symmetry: C1
Reconstruction software: Relion
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)