EMDB EMD-30310

Helical reconstruction
3.43Å resolution

Human RAD51 post-synaptic complexes mutant (V273P, D274G)

Map released:
Last modified:
Overview of EMD-30310
Source organism: Homo sapiens [9606]
Fitted atomic model: 7c9a
Primary publication:
Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures
Luo SC, Yeh HY
Nat Commun - (2020)

Function and Biology Details

Sample name: RAD51-dsDNA filament (V273P D274G mutant)
Ligands: CALCIUM ION, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
Nucleic acids: DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*T)-3'), DNA (5'-D(P*AP*AP*AP*AP*AP*AP*AP*AP*A)-3')
Proteins: RAD51-dsDNA filament (V273P D274G mutant), DNA repair protein RAD51 homolog 1

Experimental Information Details

Resolution: 3.43Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 QUANTUM (4k x 4k)