EMDB EMD-30215

Single particle reconstruction
3.14Å resolution

Mature 50S ribosomal subunit from RrmJ knock out E.coli strain

Map released:
Last modified:
Overview of EMD-30215
Source organisms:
Fitted atomic model: 7bv8
Related EM entries by publication: EMD-30211, EMD-30212, EMD-30213, EMD-30214
Primary publication:
Loss of a single methylation in 23S rRNA delays 50S assembly at multiple late stages and impairs translation initiation and elongation.
Wang W, Li W, Ge X, Yan K, Mandava CS, Sanyal S, Gao N
Proc.Natl.Acad.Sci.USA - (2020)
PMID: 32571953

Function and Biology Details

Sample name: Mature 50S ribosomal subunit from RrmJ knock out E.coli strain
Nucleic acids: 23S rRNA, 5S rRNA
Proteins: Mature 50S ribosomal subunit from RrmJ knock out E.coli strain, 50S ribosomal protein L2, 50S ribosomal protein L3, 50S ribosomal protein L4, 50S ribosomal protein L5, 50S ribosomal protein L6, 50S ribosomal protein L9, 50S ribosomal protein L10, 50S ribosomal protein L11, 50S ribosomal protein L13, 50S ribosomal protein L14, 50S ribosomal protein L15, 50S ribosomal protein L16, 50S ribosomal protein L17, 50S ribosomal protein L18, 50S ribosomal protein L19, 50S ribosomal protein L20, 50S ribosomal protein L21, 50S ribosomal protein L22, 50S ribosomal protein L23, 50S ribosomal protein L24, 50S ribosomal protein L25, 50S ribosomal protein L27, 50S ribosomal protein L28, 50S ribosomal protein L29, 50S ribosomal protein L30, 50S ribosomal protein L32, 50S ribosomal protein L33, 50S ribosomal protein L34, 50S ribosomal protein L35, 50S ribosomal protein L36

Experimental Information Details

Resolution: 3.14Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)