EMDB EMD-30214

Single particle reconstruction
3.64Å resolution

State 1 of pre50SH ribosome from RrmJ knock out E.coli stain

Map released:
Last modified:
Overview of EMD-30214
Source organism: Escherichia coli [562]
Related EM entries by publication: EMD-30211, EMD-30212, EMD-30213, EMD-30215
Primary publication:
Loss of a single methylation in 23S rRNA delays 50S assembly at multiple late stages and impairs translation initiation and elongation.
Wang W, Li W, Ge X, Yan K, Mandava CS, Sanyal S, Gao N
Proc.Natl.Acad.Sci.USA - (2020)
PMID: 32571953

Function and Biology Details

Sample name: State 1 of pre50SH ribosome
Protein: State 1 of pre50SH ribosome

Experimental Information Details

Resolution: 3.64Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)