EMDB EMD-30119

Single particle reconstruction
3.1Å resolution

CryoEM structure of Thermus thermophilus RNA polymerase elongation complex

Map released:
Overview of EMD-30119
Fitted atomic model: 6m6c
Primary publication:
Structural basis of Mfd-dependent transcription termination.
Shi J, Wen A, Zhao M, Jin S, You L, Shi Y, Dong S, Hua X, Zhang Y, Feng Y
Nucleic Acids Res. - (2020)

Function and Biology Details

Sample name: Thermus thermophilus RNA polymerase elongation complex
Ligands: ZINC ION, MAGNESIUM ION
Nucleic acids: RNA, nontemplate strand DNA, template strand DNA
Proteins: Thermus thermophilus RNA polymerase elongation complex, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', DNA-directed RNA polymerase subunit omega

Experimental Information Details

Resolution: 3.1Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)