EMDB EMD-30118

Single particle reconstruction
4.1Å resolution

Cryo-EM structure of Thermus thermophilus Mfd in complex with RNA polymerase and ATP-gamma-S

Map released:
Overview of EMD-30118
Fitted atomic model: 6m6b
Primary publication:
Structural basis of Mfd-dependent transcription termination.
Shi J, Wen A, Zhao M, Jin S, You L, Shi Y, Dong S, Hua X, Zhang Y, Feng Y
Nucleic Acids Res. - (2020)

Function and Biology Details

Sample name: Thermus thermophilus transcription-repair coupling complex
Ligands: ZINC ION, MAGNESIUM ION, PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER
Nucleic acids: template strand DNA, nontemplate strand DNA
Proteins: Thermus thermophilus transcription-repair coupling complex, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', DNA-directed RNA polymerase subunit omega, Transcription-repair-coupling factor

Experimental Information Details

Resolution: 4.1Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)