EMDB EMD-2995

Single particle reconstruction
22.3Å resolution

Negative stain 3D reconstruction of the yeast 26S proteasome in complex with ubiquitin-bound Ubp6

Map released:
Last modified:
Overview of EMD-2995
Source organism: Saccharomyces cerevisiae [4932]
Related EM entry by publication: EMD-6334
Primary publication:
Ubp6 deubiquitinase controls conformational dynamics and substrate degradation of the 26S proteasome
Bashore C, Dambacher CM, Goodall EA, Matyskiela ME, Lander GC, Martin A
NAT.STRUCT.MOL.BIOL. 22 712-719 (2015)
PMID: 26301997

Function and Biology Details

Sample name: Yeast 26S proteasome in complex with ubiquitin-bound Ubp6
Proteins: 26S proteasome, ubiquitin-bound Ubp6

Experimental Information Details

Resolution: 22.3Å
Resolution method: FSC 0.143, gold-standard
Applied symmetry: C2
Reconstruction software: Relion
Microscope: FEI TECNAI SPIRIT
Detector: TVIPS TEMCAM-F416 (4k x 4k)