EMDB › EMD-2984

Single particle reconstruction
2.2Å resolution

2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor

Map released:
Last modified:
Overview of EMD-2984
Source organisms:
Fitted atomic model: 5a1a
Primary publication:
2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor
Bartesaghi A, Merk A, Banerjee S, Matthies D, Wu X, Milne JL, Subramaniam S
SCIENCE 348 1147-1151 (2015)
PMID: 25953817

Function and Biology Details

Sample name: Escherichia coli beta-galactosidase bound to phenylethyl beta-D-thiogalactopyranoside (PETG)
Ligand: phenylethyl beta-D-thiogalactopyranoside
Protein: beta-galactosidase

Experimental Information Details

Resolution: 2.2Å
Resolution method: FSC 0.143, semi-independent
Applied symmetry: D2
Reconstruction software: FREALIGN
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)
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