EMDB EMD-2847

Single particle reconstruction
2.9Å resolution

2.9A structure of E. coli ribosome-EF-Tu complex by Cs-corrected cryo-EM

Map released:
Last modified:
Overview of EMD-2847
Source organism: Escherichia coli [562]
Fitted atomic model: 5afi
Primary publication:
Structure of the E. coli ribosome-EF-Tu complex at <3 A resolution by Cs-corrected cryo-EM
Fischer N, Neumann P, Konevega AL, Bock LV, Ficner R, Rodnina MV, Stark H
NATURE 520 567-570 (2015)
PMID: 25707802

Function and Biology Details

Sample name: E. coli 70S-EF-Tu-GDP-kirromycin-Phe-tRNAPhe-fMet-tRNAfmet-tRNAfMet complex
Nucleic acids: Phe-tRNAPhe, initiator fMet-tRNAfMet, deacylated tRNAfMet, mRNA
Protein: elongation factor Tu
Ribosome: 70S ribosome

Experimental Information Details

Resolution: 2.9Å
Resolution method: FSC 0.143, gold-standard
Applied symmetry: C1
Reconstruction software: custom-made, IMAGIC-5, Relion, 1.2
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON I (4k x 4k)