EMD-27144

Single-particle
2.66 Å
EMD-27144
3D View Gallery
Deposition: 30/05/2022
Map released: 20/12/2023
Last modified: 04/06/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-27144

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-8d2o summary report (pdf.gz) Map-8d2o FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Structure of Acidothermus cellulolyticus Cas9 ternary complex (Post-cleavage 2)

EMD-27144

Single-particle
2.66 Å
EMD-27144
3D View Gallery
Deposition: 30/05/2022
Map released: 20/12/2023
Last modified: 04/06/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Acidothermus cellulolyticus 11B, synthetic construct
Sample: CryoEM Structure of AceCas9 (Post-cleavage 2)
Fitted models: 8d2o

Deposition Authors: Rai J, Das A , Li H
Coupled catalytic states and the role of metal coordination in Cas9.
Das A , Rai J, Roth MO , Shu Y, Medina ML , Barakat MR, Li H
(2023) Nat Catal , 6 , 969 - 977
PUBMED: 38348449
DOI: doi:10.1038/s41929-023-01031-1
ISSN: 2520-1158