Single particle reconstruction
8.9Å resolution

Visualizing GroEL/ES in the Act of Encapsulating a Non-Native Substrate Protein

Map released:
Last modified:
Overview of EMD-2325
Source organisms:
Fitted atomic model: 3zpz
Related EM entry by publication: EMD-2326, EMD-2327
Primary publication:
Visualizing GroEL/ES in the act of encapsulating a folding protein.
Chen D-H, Madan D, Weaver J, Lin Z, Schroder GF, Chiu W, Rye HS
CELL(CAMBRIDGE,MASS.) 153 1354-1365 (2013)
PMID: 23746846

Function and Biology Details

Sample name: GroEL variant EL43Py capped by GroES with the assistance of nucleotide ATP
Ligand: Adenosine triphosphate
Proteins: GroELcys0-D398A-S43C-pyrene, GroES

Experimental Information Details

Resolution: 8.9Å
Resolution method: FSC 0.143, gold-standard
Applied symmetry: C7
Reconstruction software: EMAN1
Microscope: JEOL 3000SFF
Detector: KODAK SO-163 FILM