EMDB EMD-23036

Single particle reconstruction
3.5Å resolution

CryoEM structure of Yeast TFIIK (Kin28/Ccl1/Tfb3) Complex

Map released:
Overview of EMD-23036
Source organisms:
Fitted atomic model: 7kue
Primary publication:
Structure of TFIIK for phosphorylation of CTD of RNA polymerase II.
van Eeuwen T, Li T, Kim HJ, Gorbea Colon JJ, Parker MI, Dunbrack RL, Garcia BA, Tsai KL, Murakami K
Sci Adv 7 - (2021)
PMID: 33827808

Function and Biology Details

Sample name: Ternary complex of Kin28-Ccl1-Tfb3 from Saccharomyces cerevisiae.
Ligands: ADENOSINE-5'-DIPHOSPHATE, ALUMINUM FLUORIDE
Proteins: Ternary complex of Kin28-Ccl1-Tfb3 from Saccharomyces cerevisiae., RNA polymerase II transcription factor B subunit 3, Serine/threonine-protein kinase KIN28, Cyclin CCL1

Experimental Information Details

Resolution: 3.5Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: OTHER