EMDB EMD-22967

Single particle reconstruction
16.8Å resolution

Negative stain electron microscopy reconstruction of 2P SARS-CoV-2 spike ectodomain in the 3-RBD-down state after cold-denaturation at 4 degrees C for one week followed by re-naturation at 37 degrees C for 3 hours.

Map released:
Overview of EMD-22967
Related EM entry by publication: EMD-22934, EMD-22968
Primary publication:
Cold sensitivity of the SARS-CoV-2 spike ectodomain.
Edwards RJ, Mansouri K, Stalls V, Manne K, Watts B, Parks R, Gobeil SMC, Janowska K, Li D, Lu X, Deyton M, Sprenz J, Williams W, Saunders K, Sempowski GD, Henderson R, Alam M, Haynes BF, Acharya P
Biorxiv - (2020)
PMID: 32699852

Function and Biology Details

Sample name: 2P SARS-CoV-2 spike ectodomain trimer
Proteins: 2P SARS-CoV-2 spike ectodomain trimer, 2P SARS-CoV-2 spike ectodomain

Experimental Information Details

Resolution: 16.8Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI/PHILIPS EM420
Detector: OTHER