EMDB EMD-22681

Single particle reconstruction
2.9Å resolution

SARS-COV-2 nsp1 in complex with human 40S ribosome

Map released:
Overview of EMD-22681
Source organisms:
Fitted atomic model: 7k5i
Primary publication:
SARS-CoV-2 Nsp1 suppresses host but not viral translation through a bipartite mechanism.
Shi M, Wang L, Fontana P, Vora S, Zhang Y, Fu TM, Lieberman J, Wu H
Biorxiv - (2020)
PMID: 32995777

Function and Biology Details

Sample name: complex of SARS-CoV-2 Nsp1 and human 40S ribosome
Ligands: MAGNESIUM ION, ZINC ION
Nucleic acid: 40S ribosomal rRNA18S
Proteins: complex of SARS-CoV-2 Nsp1 and human 40S ribosome, human 40S ribosome, SARS-CoV-2 Nsp1, 40S ribosomal protein S17, 40S ribosomal protein S29, 40S ribosomal protein S3, 40S ribosomal protein S5, 40S ribosomal protein S10, 40S ribosomal protein S12, 40S ribosomal protein S15, 40S ribosomal protein S16, 40S ribosomal protein S18, 40S ribosomal protein S19, 40S ribosomal protein S20, 40S ribosomal protein S25, 40S ribosomal protein S28, 40S ribosomal protein S27a, Receptor of activated protein C kinase 1, 40S ribosomal protein SA, 40S ribosomal protein S3a, 40S ribosomal protein S2, 40S ribosomal protein S4, X isoform, 40S ribosomal protein S6, 40S ribosomal protein S7, 40S ribosomal protein S8, 40S ribosomal protein S9, 40S ribosomal protein S11, 40S ribosomal protein S13, 40S ribosomal protein S14, 40S ribosomal protein S21, 40S ribosomal protein S15a, 40S ribosomal protein S23, 40S ribosomal protein S24, 40S ribosomal protein S26, 40S ribosomal protein S27, 40S ribosomal protein S30, 60S ribosomal protein L41, SARS-COV-2 Nsp1

Experimental Information Details

Resolution: 2.9Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: OTHER