EMDB EMD-22506

Single particle reconstruction
3.3Å resolution

SARS-CoV-2 spike in complex with the S2A4 neutralizing antibody Fab fragment

Map released:
Overview of EMD-22506
Fitted atomic model: 7jvc
Primary publication:
Mapping Neutralizing and Immunodominant Sites on the SARS-CoV-2 Spike Receptor-Binding Domain by Structure-Guided High-Resolution Serology.
Piccoli L, Park YJ, Tortorici MA, Czudnochowski N, Walls AC, Beltramello M, Silacci-Fregni C, Pinto D, Rosen LE, Bowen JE, Acton OJ, Jaconi S, Guarino B, Minola A, Zatta F, Sprugasci N, Bassi J, Peter A, De Marco A, Nix JC, Mele F, Jovic S, Rodriguez BF, Gupta SV, Jin F, Piumatti G, Lo Presti G, Pellanda AF, Biggiogero M, Tarkowski M, Pizzuto MS, Cameroni E, Havenar-Daughton C, Smithey M, Hong D, Lepori V, Albanese E, Ceschi A, Bernasconi E, Elzi L, Ferrari P, Garzoni C, Riva A, Snell G, Sallusto F, Fink K, Virgin HW, Lanzavecchia A, Corti D, Veesler D
Cell(Cambridge,Mass.) - (2020)
PMID: 32991844

Function and Biology Details

Sample name: SARS-CoV-2 spike in complex with the S2A4 neutralizing antibody Fab fragment
Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
Proteins: SARS-CoV-2 spike in complex with the S2A4 neutralizing antibody Fab fragment, spike glycoprotein, S2A4 neutralizing antibody Fab fragment, SARS-CoV-2 spike glycoprotein, S2A4 Fab heavy chain, S2A4 Fab light chain

Experimental Information Details

Resolution: 3.3Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C3
Reconstruction software: cryoSPARC
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)