EMDB EMD-22409

Single particle reconstruction
3.3Å resolution

2:1 cGAS-nucleosome complex

Map released:
Last modified:
Overview of EMD-22409
Source organisms:
Fitted atomic model: 7joa
Related EM entry by publication: EMD-22408, EMD-22413
Primary publication:
Structural basis of nucleosome-dependent cGAS inhibition.
Boyer JA, Spangler CJ, Strauss JD, Cesmat AP, Liu P, McGinty RK, Zhang Q
Science - (2020)
PMID: 32913000

Function and Biology Details

Sample name: 2:1 cGAS-nucleosome complex
Ligand: ZINC ION
Nucleic acids: DNA (145-MER), DNA (145-MER)
Proteins: 2:1 cGAS-nucleosome complex, Histone H3.2, Histone H4, Histone H2A type 1, Histone H2B type 1-C/E/F/G/I, Cyclic GMP-AMP synthase

Experimental Information Details

Resolution: 3.3Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C2
Reconstruction software: RELION
Microscope: FEI TALOS ARCTICA
Detector: OTHER