EMDB EMD-22206

Single particle reconstruction
6.8Å resolution

Two mouse cGAS catalytic domain binding to human assembled nucleosome

Map released:
Last modified:
Overview of EMD-22206
Source organisms:
Fitted atomic model: 6xjd
Related EM entry by publication: EMD-22046, EMD-22047
Primary publication:
The Molecular Basis of Tight Nuclear Tethering and Inactivation of cGAS
Zhao B, Xu P, Rowlett CM, Jing T, Shinde O, Lei Y, West AP, Liu WR, Li P
Nature - (2020)
PMID: 32911481

Function and Biology Details

Sample name: cGAS-nucleosome complex
Ligand: ZINC ION
Nucleic acids: DNA (145-MER), DNA (145-MER)
Proteins: cGAS-nucleosome complex, Histone, DNA, Cyclic GMP-AMP synthase, Histone H3.2, Histone H4, Histone H2A type 1, Histone H2B type 1-C/E/F/G/I, Cyclic GMP-AMP synthase

Experimental Information Details

Resolution: 6.8Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Detector: GATAN K2 SUMMIT (4k x 4k)