EMDB EMD-22047

Single particle reconstruction
4.36Å resolution

The mouse cGAS catalytic domain binding to human nucleosome that purified from HEK293T cells

Map released:
Last modified:
Overview of EMD-22047
Source organisms:
Fitted atomic model: 6x5a
Related EM entry by publication: EMD-22046, EMD-22206
Primary publication:
The Molecular Basis of Tight Nuclear Tethering and Inactivation of cGAS.
Zhao B, Xu P, Rowlett CM, Jing T, Shinde O, Lei Y, West AP, Liu WR, Li P
Nature - (2020)
PMID: 32911481

Function and Biology Details

Sample name: cGAS-nucleosome complex
Ligand: ZINC ION
Nucleic acids: DNA (natural), DNA (natural)
Proteins: cGAS-nucleosome complex, Histone H3.2, Histone H4, Histone H2A type 1, Histone H2B type 1-C/E/F/G/I, Cyclic GMP-AMP synthase

Experimental Information Details

Resolution: 4.36Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)