EMDB EMD-21920

Single particle reconstruction
3.36Å resolution

Cryo-EM structure of Bacillus subtilis RNA Polymerase elongation complex

Map released:
Last modified:
Overview of EMD-21920
Fitted atomic model: 6wvj
To be published

Function and Biology Details

Sample name: DNA-directed RNA polymerase elongation complex
Ligands: ZINC ION, MAGNESIUM ION
Nucleic acids: DNA (5'-D(*TP*GP*TP*CP*GP*GP*GP*CP*GP*TP*CP*CP*GP*CP*GP*CP*GP*CP*C)-3'), RNA (5'-R(P*GP*GP*CP*GP*CP*GP*CP*G)-3'), DNA (5'-D(P*AP*CP*GP*CP*CP*CP*GP*AP*CP*A)-3')
Proteins: DNA-directed RNA polymerase elongation complex, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', DNA-directed RNA polymerase subunit omega/DNA Complex, DNA, RNA, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', DNA-directed RNA polymerase subunit omega

Experimental Information Details

Resolution: 3.36Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)