EMDB EMD-21919

Single particle reconstruction
3.6Å resolution

14-protofilament, GMPCPP stabilized microtubule with nucleotide free kinesin

Map released:
Overview of EMD-21919
Source organism: Bos taurus [9913]
Primary publication:
Dynamic and asymmetric fluctuations in the microtubule wall captured by high-resolution cryo-electron microscopy
Debs GE, Cha M, Liu X, Huehn AR, Sindelar CV
PNAS - ()

Function and Biology Details

Sample name: Microtubules stabilized with GMPCPP, fully decorated with kinesin in the nucleotide free state
Cellular component: Microtubules stabilized with GMPCPP, fully decorated with kinesin in the nucleotide free state
Proteins: alpha-Tubulin, beta-Tubulin

Experimental Information Details

Resolution: 3.6Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)