EMD-21904

Single-particle
3.16 Å
EMD-21904
3D View Gallery
Deposition: 04/05/2020
Map released: 16/09/2020
Last modified: 06/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-21904
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6wu3 summary report (pdf.gz) Map and 6wu3 FULL report (pdf.gz)
EMICSS annotations (xml)

Structure of VcINDY-Na+ in amphipol

EMD-21904

Single-particle
3.16 Å
EMD-21904
3D View Gallery
Deposition: 04/05/2020
Map released: 16/09/2020
Last modified: 06/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277 K
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TALOS ARCTICA
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.7 mm
Nominal defocus: 1.0 µm - 2.5 µm
Nominal magnification: 36000.0
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Number of real images: 1670
Average exposure time: 12.0 s
Average electron dose per image: 40.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.16 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 192836
⌯ Applied Symmetry
Point group: C2
Software [1]
Name Version Details
CryoSPARC 2.12 -
Startup model [1]
Type: OTHER
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
CryoSPARC 2.12 -
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
CryoSPARC 2.12 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: VcINDY-Na+ in amphipol
⬡ Geometry
X Y Z
Dimensions 384 384 384
Origin 0 0 0
Spacing 384 384 384
Voxel size 0.571 Å 0.571 Å 0.571 Å
Contour list
Primary Level Source
True 7.01 AUTHOR