EMDB EMD-21883

Single particle reconstruction
3.58Å resolution

Escherichia coli RNA polymerase and rrnBP1 promoter pre-open complex with DksA/ppGpp

Map released:
Overview of EMD-21883
Source organism: Escherichia coli K-12 [83333]
Fitted atomic model: 6wrg
To be published

Function and Biology Details

Sample name: Escherichia coli RNA polymerase and rrnBP1 promoter pre-open complex with DksA/ppGpp
Ligands: CHAPSO, MAGNESIUM ION, ZINC ION, GUANOSINE-5',3'-TETRAPHOSPHATE
Nucleic acids: DNA (80-MER), DNA (80-MER)
Proteins: Escherichia coli RNA polymerase and rrnBP1 promoter pre-open complex with DksA/ppGpp, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', DNA-directed RNA polymerase subunit omega, RNA polymerase sigma factor RpoD, RNA polymerase-binding transcription factor DksA, DNA, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', DNA-directed RNA polymerase subunit omega, RNA polymerase sigma factor RpoD, RNA polymerase-binding transcription factor DksA

Experimental Information Details

Resolution: 3.58Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: OTHER