EMDB EMD-21856

Single particle reconstruction
3.1Å resolution

50S ribosomal subunit without free 5S rRNA and perturbed PTC

Map released:
Overview of EMD-21856
Source organism: Escherichia coli [562]
Fitted atomic model: 6wnt
Related EM entry by publication: EMD-21857, EMD-21858
Primary publication:
Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly.
Huang S, Aleksashin NA, Loveland AB, Klepacki D, Reier K, Kefi A, Szal T, Remme J, Jaeger L, Vazquez-Laslop N, Korostelev AA, Mankin AS
Nat Commun 11 2900-2900 (2020)
PMID: 32518240

Function and Biology Details

Sample name: 50S ribosomal subunit without free 5S rRNA and perturbed PTC
Nucleic acid: 23s-5s joint ribosomal RNA
Proteins: 50S ribosomal subunit without free 5S rRNA and perturbed PTC, 50S ribosomal protein L2, 50S ribosomal protein L3, 50S ribosomal protein L4, 50S ribosomal protein L5, 50S ribosomal protein L6, 50S ribosomal protein L9, 50S ribosomal protein L10, 50S ribosomal protein L11, 50S ribosomal protein L13, 50S ribosomal protein L14, 50S ribosomal protein L15, 50S ribosomal protein L17, 50S ribosomal protein L18, 50S ribosomal protein L19, 50S ribosomal protein L20, 50S ribosomal protein L21, 50S ribosomal protein L22, 50S ribosomal protein L23, 50S ribosomal protein L24, 50S ribosomal protein L25, 50S ribosomal protein L27, 50S ribosomal protein L28, 50S ribosomal protein L29, 50S ribosomal protein L30, 50S ribosomal protein L31, 50S ribosomal protein L32, 50S ribosomal protein L34, 50S ribosomal protein L35, 50S ribosomal protein L36, 50S ribosomal protein L1

Experimental Information Details

Resolution: 3.1Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: FREALIGN
Microscope: TFS TALOS
Detector: OTHER