EMDB EMD-21685

Single particle reconstruction
3.3Å resolution

Structure of human GABA(B) receptor in an inactive state

Map released:
Last modified:
Overview of EMD-21685
Source organism: Homo sapiens [9606]
Fitted atomic model: 6wiv
Primary publication:
Structure of human GABABreceptor in an inactive state.
Park J, Fu Z, Frangaj A, Liu J, Mosyak L, Shen T, Slavkovich VN, Ray KM, Taura J, Cao B, Geng Y, Zuo H, Kou Y, Grassucci R, Chen S, Liu Z, Lin X, Williams JP, Rice WJ, Eng ET, Huang RK, Soni RK, Kloss B, Yu Z, Javitch JA, Hendrickson WA, Slesinger PA, Quick M, Graziano J, Yu H, Fiehn O, Clarke OB, Frank J, Fan QR
Nature - (2020)
PMID: 32581365

Function and Biology Details

Sample name: Heterodimer human GABA(B) receptor composed of GABA(B1b) and GABA(B2) subunits.
Ligands: N-ACETYL-D-GLUCOSAMINE, CALCIUM ION, (2R)-3-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-{[(9Z)-octadec-9-enoyl]oxy}propyl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate, CHOLESTEROL, [(2R)-3-[(Z)-icos-11-enoyl]oxy-2-[(Z)-octadec-9-enoyl]oxypropyl] 2-(trimethylazaniumyl)ethyl phosphate
Proteins: Heterodimer human GABA(B) receptor composed of GABA(B1b) and GABA(B2) subunits., Gamma-aminobutyric acid type B receptor subunit 1, Gamma-aminobutyric acid type B receptor subunit 2

Experimental Information Details

Resolution: 3.3Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: cryoSPARC
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)