EMDB EMD-21583

Single particle reconstruction
4.2Å resolution

+3 extended HIV-1 reverse transcriptase initiation complex core (pre-translocation state)

Map released:
Overview of EMD-21583
Source organisms:
Fitted atomic model: 6wb0
Primary publication:
Distinct Conformational States Underlie Pausing during Initiation of HIV-1 Reverse Transcription.
Larsen KP, Choi J, Jackson LN, Kappel K, Zhang J, Ha B, Chen DH, Puglisi EV
J.Mol.Biol. - (2020)
PMID: 32512005

Function and Biology Details

Sample name: +3 extended HIV-1 reverse transcriptase initiation complex core (pre-translocation state)
Nucleic acids: HIV-1 viral RNA genome fragment, tRNA lysine 3
Proteins: +3 extended HIV-1 reverse transcriptase initiation complex core (pre-translocation state), HIV-1 reverse transcriptase, HIV-1 RNA genome fragment, tRNA lysine 3, Reverse transcriptase/ribonuclease H, reverse transcriptase p51 subunit

Experimental Information Details

Resolution: 4.2Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)