EMDB EMD-21562

Single particle reconstruction
2.9Å resolution

MultiBody Refinement of 70S Ribosome from Enterococcus faecalis

Map released:
Overview of EMD-21562
Source organism: Enterococcus faecalis OG1RF [474186]
Fitted atomic model: 6w6p
Related EM entries by publication: EMD-0656, EMD-0657, EMD-0658, EMD-0659, EMD-0660
Primary publication:
Cryo-electron microscopy structure of the 70S ribosome from Enterococcus faecalis.
Murphy EL, Singh KV, Avila B, Kleffmann T, Gregory ST, Murray BE, Krause KL, Khayat R, Jogl G
Sci Rep 10 16301-16301 (2020)
PMID: 33004869

Function and Biology Details

Sample name: 70S ribosome
Ligand: ZINC ION
Nucleic acids: 16S rRNA, 23S rRNA, 5S rRNA
Proteins: 70S ribosome, 30S ribosomal protein S3, 30S ribosomal protein S4, 30S ribosomal protein S5, 30S ribosomal protein S6, 30S ribosomal protein S7, 30S ribosomal protein S8, 30S ribosomal protein S9, 30S ribosomal protein S10, 30S ribosomal protein S11, 30S ribosomal protein S12, 30S ribosomal protein S13, 30S ribosomal protein S14 type Z, 30S ribosomal protein S15, 30S ribosomal protein S16, 30S ribosomal protein S17, 30S ribosomal protein S18, 30S ribosomal protein S19, 30S ribosomal protein S20, 50S ribosomal protein L2, 50S ribosomal protein L3, 50S ribosomal protein L4, 50S ribosomal protein L5, 50S ribosomal protein L6, 50S ribosomal protein L13, 50S ribosomal protein L14, 50S ribosomal protein L15, 50S ribosomal protein L16, 50S ribosomal protein L17, 50S ribosomal protein L18, 50S ribosomal protein L19, 50S ribosomal protein L20, 50S ribosomal protein L21, 50S ribosomal protein L22, 50S ribosomal protein L23, 50S ribosomal protein L24, 50S ribosomal protein L27, 50S ribosomal protein L28, 50S ribosomal protein L29, 50S ribosomal protein L30, 50S ribosomal protein L32, 50S ribosomal protein L33, 50S ribosomal protein L34, 50S ribosomal protein L35, 50S ribosomal protein L36

Experimental Information Details

Resolution: 2.9Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: cryoSPARC
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 QUANTUM (4k x 4k)