EMDB EMD-21517

Single particle reconstruction
4.2Å resolution

Cryo-EM structure of anti-CRISPR AcrIF9 and dsDNA, bound to the type I-F crRNA-guided CRISPR surveillance complex

Map released:
Last modified:
Overview of EMD-21517
Source organisms:
Fitted atomic model: 6whi
Related EM entry by publication: EMD-21516
Primary publication:
AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex.
Hirschi M, Lu WT, Santiago-Frangos A, Wilkinson R, Golden SM, Davidson AR, Lander GC, Wiedenheft B
Nat Commun 11 2730-2730 (2020)
PMID: 32483187

Function and Biology Details

Sample name: Anti-CRISPR AcrIF9 bound to the type I-F crRNA-guided CRISPR surveillance complex
Proteins: Anti-CRISPR AcrIF9 bound to the type I-F crRNA-guided CRISPR surveillance complex, type I-F crRNA-guided CRISPR surveillance complex, Anti-CRISPR AcrIF9, CRISPR-associated protein Csy1, Type I-F CRISPR-associated protein Csy2, CRISPR-associated protein Csy3, RNA (60-MER), CRISPR-associated endonuclease Cas6/Csy4, anti-CRISPR AcrIF9, non-target dsDNA, non-target dsDNA

Experimental Information Details

Resolution: 4.2Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TALOS ARCTICA
Detector: GATAN K2 SUMMIT (4k x 4k)