EMDB < EMD-21039

EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

Services:

offer vital services to scientists in the member states

Training

scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

EMD-21039

Tomography
12.28 Å

EMD-21039

Gallery

Deposition: 25/11/2019
Map released: 27/05/2020
Last modified: 17/06/2020

Overview Sample Experiment Validation Additional data Links

Overview 3D View Sample Experiment Validation Additional data Links

EMD-21039

Cryo-electron tomogram of FIB-milled U2OS cells

EMD-21039

Tomography
12.28 Å
EMD-21039

EMD-21039

Gallery

Deposition: 25/11/2019
Map released: 27/05/2020
Last modified: 17/06/2020

Overview Sample Experiment Validation Additional data Links

Sample Organism: Homo sapiens
Sample: FIB-milled frozen human osteosarcoma U2OS cell

Deposition Authors: Watanabe R, Villa E

Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography.

Wagner FR, Watanabe R , Schampers R, Singh D, Persoon H, Schaffer M, Fruhstorfer P, Plitzko J, Villa E
(2020) Nat Protoc , 15 , 2041 - 2070

PUBMED: 32405053
DOI: doi:10.1038/s41596-020-0320-x
ISSN: 1750-2799

Grant Support:

National Institutes of Health/Office of the Director: 1DP2GM123494-01 (United States)
National Science Foundation (NSF, United States): ECCS-1542148 (United States)