EMDB EMD-20833

Single particle reconstruction
3.5Å resolution

Cryo-EM reconstruction of the PrgHK periplasmic ring from Salmonella's needle complex assembled in the absence of the export apparatus

Map released:
Overview of EMD-20833
Fitted atomic model: 6uov
Related EM entries by publication: EMD-20830, EMD-20831, EMD-20832, EMD-20838
Primary publication:
High-resolution view of the type III secretion export apparatus in situ reveals membrane remodeling and a secretion pathway.
Butan C, Lara-Tejero M, Li W, Liu J, Galan JE
Proc.Natl.Acad.Sci.USA 116 24786-24795 (2019)
PMID: 31744874

Function and Biology Details

Sample name: Complex of the periplasmic domains of PrgH and PrgK from Salmonella's needle complex assembled in the absence of the export apparatus
Proteins: Complex of the periplasmic domains of PrgH and PrgK from Salmonella's needle complex assembled in the absence of the export apparatus, Lipoprotein PrgK, Protein PrgH

Experimental Information Details

Resolution: 3.5Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C23
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)