EMDB EMD-20832

Single particle reconstruction
3.3Å resolution

Cryo-EM structure of the PrgHK periplasmic ring from the Salmonella SPI-1 type III secretion needle complex solved at 3.3 angstrom resolution

Map released:
Overview of EMD-20832
Fitted atomic model: 6uot
Related EM entries by publication: EMD-20830, EMD-20831, EMD-20833, EMD-20838
Primary publication:
High-resolution view of the type III secretion export apparatus in situ reveals membrane remodeling and a secretion pathway.
Butan C, Lara-Tejero M, Li W, Liu J, Galan JE
Proc.Natl.Acad.Sci.USA 116 24786-24795 (2019)
PMID: 31744874

Function and Biology Details

Sample name: Complex of the periplasmic domains of PrgH and PrgK from the Salmonella SPI-1 type III secretion needle complex
Proteins: Complex of the periplasmic domains of PrgH and PrgK from the Salmonella SPI-1 type III secretion needle complex, Protein PrgH, Lipoprotein PrgK

Experimental Information Details

Resolution: 3.3Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C24
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)