EMDB EMD-20831

Single particle reconstruction
5.3Å resolution

Cryo-EM reconstruction shows that the needle complex's inner rings from Salmonella assemble with 23-fold symmetry in the absence of the export apparatus.

Map released:
Overview of EMD-20831
Related EM entries by publication: EMD-20830, EMD-20832, EMD-20833, EMD-20838
Primary publication:
High-resolution view of the type III secretion export apparatus in situ reveals membrane remodeling and a secretion pathway.
Butan C, Lara-Tejero M, Li W, Liu J, Galan JE
Proc.Natl.Acad.Sci.USA 116 24786-24795 (2019)
PMID: 31744874

Function and Biology Details

Sample name: Complex of PrgH and PrgK, the protein components of the inner rings of Salmonella's needle complex, assembled in the absence of the export apparatus.
Protein: Complex of PrgH and PrgK, the protein components of the inner rings of Salmonella's needle complex, assembled in the absence of the export apparatus.

Experimental Information Details

Resolution: 5.3Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)