EMDB EMD-20770

Single particle reconstruction
3.9Å resolution

CryoEM structure of human Arp2/3 complex with bound NPFs

Map released:
Overview of EMD-20770
Source organisms:
Fitted atomic model: 6uhc
Primary publication:
Cryo-EM structure of NPF-bound human Arp2/3 complex and activation mechanism.
Zimmet A, Van Eeuwen T, Boczkowska M, Rebowski G, Murakami K, Dominguez R
Sci Adv 6 eaaz7651-eaaz7651 (2020)
PMID: 32548263

Function and Biology Details

Sample name: Cryo-EM structure of human Arp2/3 complex with bound NPFs
Ligands: MAGNESIUM ION, ADENOSINE-5'-TRIPHOSPHATE
Proteins: Cryo-EM structure of human Arp2/3 complex with bound NPFs, Actin-related protein 3, Actin-related protein 2, Actin-related protein 2/3 complex subunit 1B, Actin-related protein 2/3 complex subunit 2, Actin-related protein 2/3 complex subunit 3, Actin-related protein 2/3 complex subunit 4, Actin-related protein 2/3 complex subunit 5, Neural Wiskott-Aldrich syndrome protein

Experimental Information Details

Resolution: 3.9Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: cryoSPARC
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)