EMDB EMD-20741

Single particle reconstruction
3.21Å resolution

Human IMPDH2 treated with ATP, IMP, and 20 mM GTP. Fully compressed filament segment reconstruction.

Map released:
Overview of EMD-20741
Source organism: Homo sapiens [9606]
Fitted atomic model: 6udo
Primary publication:
Cryo-EM structures demonstrate human IMPDH2 filament assembly tunes allosteric regulation.
Johnson MC, Kollman JM
Elife 9 - (2020)
PMID: 31999252

Function and Biology Details

Sample name: Human IMPDH2 treated with ATP, IMP, and 20 mM GTP. Filament assembly interface reconstruction.
Cellular component: Human IMPDH2 treated with ATP, IMP, and 20 mM GTP. Filament assembly interface reconstruction.
Ligands: INOSINIC ACID, GUANOSINE-5'-TRIPHOSPHATE, ADENOSINE-5'-TRIPHOSPHATE
Protein: Inosine-5'-monophosphate dehydrogenase 2

Experimental Information Details

Resolution: 3.21Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: D4
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)