EMDB EMD-20691

Single particle reconstruction
3.14Å resolution

Human IMPDH2 treated with ATP, IMP, NAD+, and 2 mM GTP. Filament assembly interface reconstruction.

Map released:
Overview of EMD-20691
Source organism: Homo sapiens [9606]
Fitted atomic model: 6u8s
Primary publication:
Cryo-EM structures demonstrate human IMPDH2 filament assembly tunes allosteric regulation.
Johnson MC, Kollman JM
Elife 9 - (2020)
PMID: 31999252

Function and Biology Details

Sample name: Human IMPDH2 treated with ATP, IMP, NAD+, and 2 mM GTP. Fully compressed filament segment reconstruction.
Cellular component: Human IMPDH2 treated with ATP, IMP, NAD+, and 2 mM GTP. Fully compressed filament segment reconstruction.
Ligands: INOSINIC ACID, NICOTINAMIDE-ADENINE-DINUCLEOTIDE
Protein: Inosine-5'-monophosphate dehydrogenase 2

Experimental Information Details

Resolution: 3.14Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: D4
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)