EMDB EMD-20607

Single particle reconstruction
3.9Å resolution

Structure of the S. cerevisiae replicative helicase CMG in complex with a forked DNA

Map released:
Overview of EMD-20607
Source organism: Saccharomyces cerevisiae [4932]
Fitted atomic model: 6u0m
Primary publication:
DNA unwinding mechanism of a eukaryotic replicative CMG helicase.
Yuan Z, Georgescu R, Bai L, Zhang D, Li H, O'Donnell ME
Nat Commun 11 688- (2020)

Function and Biology Details

Sample name: CMG-forkDNA
Ligand: ADENOSINE-5'-TRIPHOSPHATE
Nucleic acids: DNA (26-MER), DNA (15-MER)
Proteins: CMG-forkDNA, DNA replication complex GINS protein PSF1, DNA replication complex GINS protein PSF2, DNA replication complex GINS protein PSF3, DNA replication complex GINS protein SLD5, Cell division control protein 45, DNA replication licensing factor MCM2, DNA replication licensing factor MCM3, DNA replication licensing factor MCM4, Minichromosome maintenance protein 5, DNA replication licensing factor MCM6, DNA replication licensing factor MCM7

Experimental Information Details

Resolution: 3.9Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)