EMDB EMD-20508

Single particle reconstruction
3.1Å resolution

MscS Nanodisc with N-terminal His-Tag

Map released:
Overview of EMD-20508
Source organisms:
Fitted atomic model: 6pwn
Related EM entry by publication: EMD-20509, EMD-20510
Primary publication:
Molecular basis of force-from-lipids gating in the mechanosensitive channel MscS.
Reddy B, Bavi N, Lu A, Park Y, Perozo E
Elife 8 - (2019)
PMID: 31880537

Function and Biology Details

Sample name: Mechanosensitive Channel of Small Conductance
Ligands: (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate, HEXADECANE
Proteins: Mechanosensitive Channel of Small Conductance, Small-conductance mechanosensitive channel

Experimental Information Details

Resolution: 3.1Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C7
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)