EMDB EMD-20504

Subtomogram averaging
9.83Å resolution

10 Angstrom structure of the asymmetric flagellar filament purified from Leptospira biflexa Patoc WT cells resolved via subtomogram averaging

Map released:
Overview of EMD-20504
Fitted atomic model: 6pwb
Related EM entry by publication: EMD-20503
Primary publication:
An asymmetric sheath controls flagellar supercoiling and motility in the leptospira spirochete.
Gibson KH, Trajtenberg F, Wunder EA, Brady MR, San Martin F, Mechaly A, Shang Z, Liu J, Picardeau M, Ko A, Buschiazzo A, Sindelar CV
Elife 9 - (2020)
PMID: 32157997

Function and Biology Details

Sample name: Flagellar filament purified from the periplasm of the Spirochete bacterium, Leptospira biflexa
Cellular component: Flagellar filament purified from the periplasm of the Spirochete bacterium, Leptospira biflexa
Proteins: Flagellin B1 (FlaB1), Flagellar coiling protein A (FcpA), Flagellar coiling protein B (FcpB)

Experimental Information Details

Resolution: 9.83Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: emClarity
Microscope: FEI POLARA 300
Detector: GATAN K2 SUMMIT (4k x 4k)