EMD-20491

Single-particle
3.4 Å
EMD-20491
3D View Gallery
Deposition: 20/07/2019
Map released: 18/09/2019
Last modified: 28/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-20491
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6pvk summary report (pdf.gz) Map and 6pvk FULL report (pdf.gz)
EMICSS annotations (xml)

Bacterial 45SRbgA ribosomal particle class A

EMD-20491

Single-particle
3.4 Å
EMD-20491
3D View Gallery
Deposition: 20/07/2019
Map released: 18/09/2019
Last modified: 28/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Details: 20mM Tris-HCl pH 7.5, 10mM MgCl2, 50mM NH4Cl, 1mM DTT
Grid
Details: Unspecified
Support Film [1]
Material Topology Thickness
CARBON HOLEY ARRAY -
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK IV
Details: Cryo-EM grids were prepared by applying a 3.6 microliters volume of the diluted samples to holey carbon grids (c-flat CF-2/2-2C-T) with a freshly applied additional layer of continuous thin carbon (5-10nm). Grids were blotted for 3 seconds and with a blot force +1.
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Image Recording [1]
Detector model: FEI FALCON II (4k x 4k)
Detector mode: INTEGRATING
Number of real images: 8950
Average exposure time: 1.0 s
Average electron dose per image: 40.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.4 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 574260
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION 2.0 -
Startup model [1]
Type: PDB ENTRY
PDB model: 3J9W
⦨ Initial angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
RELION - -
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION - -
Particle selection [1]
Selected Ref. model Method Software Details
1339712 - - - -
Final 3D classification
Software [1]
Name Version Details
RELION - -
CTF correction
Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Software [1]
Name Version Details
Gctf - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 336 336 336
Dimensions (Å) 360.52798 360.52798 360.52798
Voxel size (Å) 1.073 1.073 1.073
Contour list
Primary Level Source
True 0.076 AUTHOR