EMDB EMD-20485

Single particle reconstruction
2.8Å resolution

Structure of HIV cleaved synaptic complex (CSC) intasome bound with magnesium and INSTI XZ446 (compound 4f)

Map released:
Last modified:
Overview of EMD-20485
Source organisms:
Fitted atomic model: 6puz
Related EM entries by publication: EMD-20481, EMD-20483, EMD-20484, EMD-21038
Primary publication:
Structural basis for strand-transfer inhibitor binding to HIV intasomes.
Passos DO, Li M, Jozwik IK, Zhao XZ, Santos-Martins D, Yang R, Smith SJ, Jeon Y, Forli S, Hughes SH, Burke Jr TR, Craigie R, Lyumkis D
Science 367 810-814 (2020)
PMID: 32001521

Function and Biology Details

Sample name: Assembly of HIV integrase and viral DNA
Ligands: MAGNESIUM ION, ZINC ION, 4-azanyl-N-[[2,4-bis(fluoranyl)phenyl]methyl]-1-oxidanyl-2-oxidanylidene-6-[2-(phenylsulfonyl)ethyl]-1,8-naphthyridine-3-carboxamide, water
Nucleic acids: viral DNA non-transferred strand, viral DNA transferred strand
Proteins: Assembly of HIV integrase and viral DNA, Chimeric Sso7d and HIV-1 integrase

Experimental Information Details

Resolution: 2.8Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C2
Reconstruction software: cisTEM
Detector: GATAN K2 SUMMIT (4k x 4k)