EMDB EMD-20470

Single particle reconstruction
3.2Å resolution

Cryo-EM structure of human cannabinoid receptor 2-Gi protein in complex with agonist WIN 55,212-2

Map released:
Overview of EMD-20470
Source organisms:
Fitted atomic model: 6pt0
Primary publication:
Cryo-EM Structure of the Human Cannabinoid Receptor CB2-GiSignaling Complex.
Xing C, Zhuang Y, Xu TH, Feng Z, Zhou XE, Chen M, Wang L, Meng X, Xue Y, Wang J, Liu H, McGuire TF, Zhao G, Melcher K, Zhang C, Xu HE, Xie XQ
Cell - (2020)
PMID: 32004460

Function and Biology Details

Sample name: human cannabinoid receptor 2-Gi protein
Ligands: {(3R)-5-methyl-3-[(morpholin-4-yl)methyl]-2,3-dihydro[1,4]oxazino[2,3,4-hi]indol-6-yl}(naphthalen-1-yl)methanone, PALMITIC ACID, CHOLESTEROL
Proteins: human cannabinoid receptor 2-Gi protein, Cannabinoid receptor 2, Guanine nucleotide-binding protein G(i) subunit alpha-1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, scFv16

Experimental Information Details

Resolution: 3.2Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)