EMDB EMD-20465

Single particle reconstruction
3.5Å resolution

Escherichia coli RNA polymerase promoter unwinding intermediate (TpreRPo) with TraR and rpsT P2 promoter

Map released:
Overview of EMD-20465
Source organism: Escherichia coli [562]
Fitted atomic model: 6psv
Related EM entries by publication: EMD-20460, EMD-20461, EMD-20462, EMD-20463, EMD-20464, EMD-20466
Primary publication:
Stepwise Promoter Melting by Bacterial RNA Polymerase.
Chen J, Chiu C, Gopalkrishnan S, Chen AY, Olinares PDB, Saecker RM, Winkelman JT, Maloney MF, Chait BT, Ross W, Gourse RL, Campbell EA, Darst SA
Mol.Cell - (2020)
PMID: 32160514

Function and Biology Details

Sample name: Escherichia coli sigma70-holoenzyme bound to TraR and Wildtype rpsT P2 as TpreRPo
Ligands: ZINC ION, CHAPSO, MAGNESIUM ION
Nucleic acids: DNA (85-MER), DNA (85-MER)
Proteins: Escherichia coli sigma70-holoenzyme bound to TraR and Wildtype rpsT P2 as TpreRPo, Protein TraR, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', DNA-directed RNA polymerase subunit omega, RNA polymerase sigma factor RpoD

Experimental Information Details

Resolution: 3.5Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)