EMDB EMD-20457

Single particle reconstruction
3.3Å resolution

Cryo-EM structure of HzTransib strand transfer complex (STC)

Map released:
Overview of EMD-20457
Source organism: Helicoverpa zea [7113]
Fitted atomic model: 6pr5
Primary publication:
Structures of a RAG-like transposase during cut-and-paste transposition
Liu C, Yang Y, Schatz DG
Nature - (2019)

Function and Biology Details

Sample name: Strand transfer complex of HzTransib with transposon ends covalently linked to target DNA.
Ligands: MAGNESIUM ION, ZINC ION
Nucleic acids: DNA (5'-D(*GP*AP*TP*CP*TP*GP*GP*CP*CP*TP*AP*GP*AP*TP*CP*TP*CP*A)-3'), DNA (5'-D(P*CP*AP*CP*GP*GP*TP*GP*GP*AP*TP*CP*GP*AP*AP*AP*A)-3'), DNA (30-MER), DNA (5'-D(P*TP*TP*TP*TP*CP*GP*AP*TP*C)-3'), DNA (39-MER)
Proteins: Strand transfer complex of HzTransib with transposon ends covalently linked to target DNA., DNA-mediated transposase

Experimental Information Details

Resolution: 3.3Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)