EMDB EMD-20456

Single particle reconstruction
4.2Å resolution

Cryo-EM structure of HzTransib/TIR DNA transposon end complex (TEC)

Map released:
Overview of EMD-20456
Source organism: Helicoverpa zea [7113]
Fitted atomic model: 6pqy
Primary publication:
Structures of a RAG-like transposase during cut-and-paste transposition
Liu C, Yang Y, Schatz DG
Nature - (2019)

Function and Biology Details

Sample name: Transposon end complex of HzTransib with cleaved TIR substrate DNA
Nucleic acids: DNA (5'-D(P*CP*AP*CP*GP*GP*TP*GP*GP*AP*TP*CP*GP*AP*AP*AP*A)-3'), DNA (5'-D(P*TP*TP*TP*TP*CP*GP*AP*TP*CP*CP*AP*CP*CP*GP*TP*G)-3')
Proteins: Transposon end complex of HzTransib with cleaved TIR substrate DNA, Putative DNA-mediated transposase

Experimental Information Details

Resolution: 4.2Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C2
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)