EMDB EMD-20441

Single particle reconstruction
4.4Å resolution

RbgA+45SRbgA complex

Map released:
Overview of EMD-20441
Source organism: Bacillus subtilis [1423]
Fitted atomic model: 6ppk
Primary publication:
RbgA Triggers Folding of the 50S Subunit Functional Sites
Seffouh A, Jain N, Jahagirdar D, Basu K, Razi A, Ni X, Guarne A, Britton RA, Ortega J
Nucleic Acids Res. - (2019)

Function and Biology Details

Sample name: RbgA + 45SRbgA ribosomal assembly intermediate complex
Ligand: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER
Nucleic acids: 23S rRNA, 5S rRNA
Proteins: RbgA + 45SRbgA ribosomal assembly intermediate complex, 50S ribosomal protein L2, 50S ribosomal protein L3, 50S ribosomal protein L4, 50S ribosomal protein L5, 50S ribosomal protein L6, 50S ribosomal protein L13, 50S ribosomal protein L14, 50S ribosomal protein L15, 50S ribosomal protein L17, 50S ribosomal protein L18, 50S ribosomal protein L19, 50S ribosomal protein L20, 50S ribosomal protein L21, 50S ribosomal protein L22, 50S ribosomal protein L23, 50S ribosomal protein L24, 50S ribosomal protein L27, 50S ribosomal protein L30, 50S ribosomal protein L32, 50S ribosomal protein L29, 50S ribosomal protein L34, Ribosome biogenesis GTPase A

Experimental Information Details

Resolution: 4.4Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TECNAI F20
Detector: GATAN K2 SUMMIT (4k x 4k)